Enzymes Trivia Questions and Answers

green and white flower buds in tilt shift lens

1: What are enzymes composed of?
A: Carbohydrates
B: Lipids
C: Proteins
D: Nucleic acids
Correct Answer: C
Explanation: Enzymes are proteins that act as biological catalysts, accelerating chemical reactions.

2: What term is used to describe molecules upon which enzymes act?
A: Catalysts
B: Products
C: Solvents
D: Substrates
Correct Answer: D
Explanation: The molecules upon which enzymes act are called substrates.

3: What process relies on enzymes to occur at rates fast enough to sustain life?
A: Photosynthesis
B: Digestion
C: Metabolic processes
D: Respiration
Correct Answer: C
Explanation: Almost all metabolic processes in the cell need enzyme catalysis to occur at rates fast enough to sustain life.

4: What is the field of study focused on enzymes called?
A: Biochemistry
B: Enzymology
C: Metabology
D: Catalysis
Correct Answer: B
Explanation: The study of enzymes is called enzymology.

5: How many types of biochemical reactions are enzymes known to catalyze?
A: 100
B: 500
C: 5,000
D: 50,000
Correct Answer: C
Explanation: Enzymes are known to catalyze more than 5,000 biochemical reaction types.

6: What type of catalytic molecules, besides proteins, are also classified as biocatalysts?
A: Lipids
B: Ribozymes
C: Hormones
D: Minerals
Correct Answer: B
Explanation: Ribozymes, catalytic RNA molecules, are sometimes described as a type of enzyme.

7: What unique structural feature gives enzymes their specificity?
A: Two-dimensional shape
B: Concentration gradient
C: Three-dimensional structure
D: Amino acid length
Correct Answer: C
Explanation: An enzyme’s specificity comes from its unique three-dimensional structure.

8: Which enzyme converts maltose into two glucose molecules?
A: Amylase
B: Lactase
C: Glucosidase
D: Protease
Correct Answer: C
Explanation: The enzyme glucosidase converts the sugar maltose into two glucose sugars.

9: Which enzyme enables a reaction that would otherwise take millions of years to occur in milliseconds?
A: Hexokinase
B: Catalase
C: Orotidine 5′-phosphate decarboxylase
D: Lipase
Correct Answer: C
Explanation: Orotidine 5′-phosphate decarboxylase allows a reaction that would otherwise take millions of years to occur in milliseconds.

10: What are molecules called that decrease enzyme activity?
A: Catalysts
B: Inhibitors
C: Coenzymes
D: Substrates
Correct Answer: B
Explanation: Inhibitors are molecules that decrease enzyme activity.

11: What happens to most enzymes when exposed to excessive heat?
A: They speed up
B: They freeze
C: They denature
D: They multiply
Correct Answer: C
Explanation: Most enzymes are permanently denatured when exposed to excessive heat, losing their structure and catalytic properties.

12: Which household product uses enzymes to break down protein, starch, or fat stains on clothes?
A: Fabric softener
B: Biological washing powder
C: Bleach
D: Detergent booster
Correct Answer: B
Explanation: Enzymes in biological washing powders break down protein, starch, or fat stains on clothes.

13: Who was the first to discover an enzyme, diastase, in 1833?
A: Louis Pasteur
B: Eduard Buchner
C: Anselme Payen
D: Wilhelm Kühne
Correct Answer: C
Explanation: French chemist Anselme Payen was the first to discover an enzyme, diastase.

14: Which term did Louis Pasteur use to describe the fermentation process?
A: Enzyme
B: Ferment
C: Catalyst
D: Substrate
Correct Answer: B
Explanation: Louis Pasteur concluded fermentation was caused by ferments, a vital force contained within yeast cells.

15: Who first used the term enzyme in 1877?
A: Eduard Buchner
B: Wilhelm Kühne
C: Anselme Payen
D: Louis Pasteur
Correct Answer: B
Explanation: German physiologist Wilhelm Kühne first used the term enzyme in 1877.

16: What enzyme did Eduard Buchner name that brought about the fermentation of sucrose?
A: Zymase
B: Amylase
C: Protease
D: Catalase
Correct Answer: A
Explanation: Eduard Buchner named the enzyme that brought about the fermentation of sucrose “zymase.”

17: For what discovery did Eduard Buchner receive the Nobel Prize in Chemistry in 1907?
A: Cell theory
B: Cell-free fermentation
C: Photosynthesis
D: Protein synthesis
Correct Answer: B
Explanation: Eduard Buchner received the Nobel Prize in Chemistry for “his discovery of cell-free fermentation”.

18: Who demonstrated that pure proteins can be enzymes by crystallizing the enzyme urease in 1926?
A: Richard Willstätter
B: Louis Pasteur
C: John Howard Northrop
D: James B. Sumner
Correct Answer: D
Explanation: James B. Sumner demonstrated that pure proteins can be enzymes by crystallizing the enzyme urease.

19: Which enzyme, found in tears, saliva, and egg whites, was the first to have its structure solved by x-ray crystallography?
A: Catalase
B: Lysozyme
C: Lipase
D: Peroxidase
Correct Answer: B
Explanation: Lysozyme was the first enzyme to have its structure solved by x-ray crystallography.

20: Which type of enzyme adds a phosphate group to a substrate?
A: Oxidoreductases
B: Transferases
C: Hydrolases
D: Ligases
Correct Answer: B
Explanation: Transferases add a functional group, such as a phosphate group, to a substrate.

21: What is the top-level classification for enzymes that catalyze oxidation/reduction reactions?
A: EC 1
B: EC 2
C: EC 3
D: EC 4
Correct Answer: A
Explanation: EC 1, Oxidoreductases, catalyze oxidation/reduction reactions.

22: Enzymes are essential for which type of biological processes in the cell?
A: Reproductive
B: Sensory
C: Metabolic
D: Neural
Correct Answer: C
Explanation: Enzymes are essential for metabolic processes in the cell.

23: What term describes unrelated enzymes that have the same enzymatic activity?
A: Isozymes
B: Allosteric enzymes
C: Non-homologous isofunctional enzymes
D: Pseudoenzymes
Correct Answer: C
Explanation: Unrelated enzymes that have the same enzymatic activity are called non-homologous isofunctional enzymes.

24: What effect does temperature have on enzyme activity?
A: No effect
B: Decreases activity
C: Initially increases then decreases activity
D: Permanently increases activity
Correct Answer: C
Explanation: Enzyme activity initially increases with temperature until the enzyme denatures, leading to a decrease in activity.

25: What is the term used for the small portions of enzyme structure directly involved in catalysis?
A: Allosteric sites
B: Active site
C: Catalytic site
D: Binding site
Correct Answer: C
Explanation: The small portions of the enzyme structure directly involved in catalysis are called the catalytic site.

26: What model describes enzyme and substrate possessing complementary geometric shapes?
A: Induced fit model
B: Substrate model
C: Lock and key model
D: Conformation model
Correct Answer: C
Explanation: The lock and key model describes enzymes and substrates possessing specific complementary geometric shapes that fit exactly into one another.

27: What alternative model suggests that the active site of enzymes continuously reshapes?
A: Lock and key model
B: Conformation adaptation model
C: Substrate model
D: Induced fit model
Correct Answer: D
Explanation: The induced fit model suggests that the active site of enzymes continuously reshapes as the substrate interacts with it.

28: What role do inhibitors play in enzymatic activity?
A: Increase enzyme production
B: Decrease enzyme activity
C: Change enzyme structure
D: Increase substrate concentration
Correct Answer: B
Explanation: Inhibitors are molecules that decrease enzyme activity.

29: What is the function of allosteric sites on enzymes?
A: Bind substrates
B: Form covalent bonds
C: Regulate enzyme activity
D: Increase reaction rates
Correct Answer: C
Explanation: Allosteric sites are pockets on the enzyme that bind molecules, causing a conformational change that affects the enzyme’s reaction rate.

30: What term is used to describe enzymes that contain multiple protein subunits?
A: Isozymes
B: Cofactors
C: Holoenzymes
D: Apoproteins
Correct Answer: C
Explanation: The term holoenzyme can be applied to enzymes that contain multiple protein subunits, such as the DNA polymerases.

31: What are small organic molecules that transport chemical groups from one enzyme to another called?
A: Catalysts
B: Cofactors
C: Coenzymes
D: Substrates
Correct Answer: C
Explanation: Coenzymes are small organic molecules that transport chemical groups from one enzyme to another.

32: How do enzymes affect the activation energy of reactions?
A: Increase it
B: Stabilize it
C: Decrease it
D: Do not affect it
Correct Answer: C
Explanation: Enzymes increase reaction rates by lowering the activation energy needed to form the transition state.

33: What is the main purpose of enzyme kinetics?
A: Produce more enzymes
B: Increase substrate synthesis
C: Stabilize transition states
D: Study how enzymes bind substrates and turn them into products
Correct Answer: D
Explanation: Enzyme kinetics investigates how enzymes bind substrates and turn them into products.

34: What condition represents the maximum reaction rate (Vmax) of an enzyme?
A: Low temperature
B: High pH
C: All enzyme active sites bound to the substrate
D: Minimum substrate concentration
Correct Answer: C
Explanation: Vmax represents the maximum reaction rate of an enzyme when all active sites are bound to the substrate.

35: What name is given to the factor by which enzyme efficiency is expressed?
A: Michaelis–Menten constant (Km)
B: Turnover number (kcat)
C: Specificity constant (kcat/Km)
D: Diffusion limit
Correct Answer: C
Explanation: Enzyme efficiency is expressed in terms of the specificity constant (kcat/Km).

36: What type of inhibitor cannot bind to the free enzyme, only to the enzyme-substrate complex?
A: Competitive
B: Non-competitive
C: Uncompetitive
D: Mixed
Correct Answer: C
Explanation: An uncompetitive inhibitor cannot bind to the free enzyme, only to the enzyme-substrate complex.

37: What common drug acts as an irreversible enzyme inhibitor?
A: Aspirin
B: Statins
C: Methotrexate
D: Protease inhibitors
Correct Answer: A
Explanation: Aspirin is a common drug that acts as an irreversible enzyme inhibitor.

38: What type of enzymes do protease inhibitors target for treating HIV infections?
A: Kinases
B: Hydrolases
C: Transferases
D: Oxidoreductases
Correct Answer: B
Explanation: Protease inhibitors target hydrolase enzymes, specifically those that cleave peptide bonds in proteins.

39: What pH range typically represents the optimal pH for the enzyme catalase?
A: 1.5-1.6
B: 4.5-5.0
C: 7.0
D: 8.0-9.0
Correct Answer: C
Explanation: The enzyme catalase has an optimal pH of around 7.0, which is neutral.

40: What is an example of an enzyme involved in signal transduction and cell regulation?
A: Protease
B: Kinase
C: Lipase
D: Amylase
Correct Answer: B
Explanation: Enzymes such as kinases are involved in signal transduction and cell regulation.

41: Which enzyme generates light in fireflies?
A: Luciferase
B: Myosin
C: DNA polymerase
D: Integrase
Correct Answer: A
Explanation: Luciferase generates light in fireflies through a bioluminescent reaction.

42: Enzymes in the digestive systems of animals break down large molecules like starch or proteins into what?
A: Lipids
B: Smaller molecules
C: Hormones
D: Ions
Correct Answer: B
Explanation: Digestive enzymes break down large molecules like starch or proteins into smaller molecules, which can then be absorbed.

43: What unique enzyme do microorganisms in the gut of ruminants produce to break down cellulose?
A: Protease
B: Amylase
C: Cellulase
D: Lipase
Correct Answer: C
Explanation: Microorganisms in the gut of ruminants produce cellulase to break down the cellulose cell walls of plant fiber.

44: What do regulatory mechanisms such as enzyme induction involve?
A: Increase enzyme degradation
B: Shrink enzyme size
C: Enhance or diminish enzyme production
D: Change enzyme’s three-dimensional shape
Correct Answer: C
Explanation: Enzyme induction involves enhancing or diminishing enzyme production in response to changes in the cell’s environment.

45: What process involves regulation by producing an inactive precursor to an enzyme?
A: Phosphorylation
B: Zymogen formation
C: Coenzyme synthesis
D: Feedback inhibition
Correct Answer: B
Explanation: Zymogen formation involves producing an inactive precursor to an enzyme, which is later activated under specific conditions.

46: How can enzyme subcellular localization be altered?
A: Changes in pH levels
B: Enzyme phosphorylation
C: Polymerisation into cytoplasmic filaments
D: Coenzyme binding
Correct Answer: C
Explanation: Enzyme subcellular localization can be altered through polymerisation of enzymes into macromolecular cytoplasmic filaments.

47: What forms the basis of enzyme evolution?
A: Metal ion binding
B: Sequence divergence and mutations
C: Constant substrate concentration
D: Stabilized enzyme active sites
Correct Answer: B
Explanation: Enzymes, like any other proteins, change over time through mutations and sequence divergence, forming the basis of enzyme evolution.

48: How do genes coding for DNA repair enzymes, when mutated, cause cancer?
A: Increasing enzyme efficiency
B: Accumulation of mutations due to poor DNA repair
C: Enhancing cell growth
D: Reducing enzyme-substrate binding
Correct Answer: B
Explanation: Defects in DNA repair enzymes cause cancer because cells become less able to repair mutations in their genomes.

49: Which enzyme deficiency leads to Tay-Sachs disease?
A: Lipase
B: Hexokinase
C: Hexosaminidase
D: Catalase
Correct Answer: C
Explanation: Tay-Sachs disease is caused by a deficiency in the enzyme hexosaminidase.

50: What term is used for enzymes sharing similar sequences but catalyzing different reactions?
A: Isozymes
B: Kinases
C: Non-homologous isofunctional enzymes
D: Transferases
Correct Answer: C
Explanation: Enzymes that have similar sequences but catalyze different reactions are called non-homologous isofunctional enzymes.

51: What key advantage do enzymes from bacteria living in volcanic environments offer for industrial users?
A: Operate at low temperatures
B: Require low pH levels
C: Function at high temperatures
D: Enhance substrate binding
Correct Answer: C
Explanation: Enzymes from bacteria living in volcanic environments can function at high temperatures, allowing industrial enzyme-catalyzed reactions to operate at a very high rate.

52: What enzyme is commonly used to increase the rate of protein digestion in meat tenderizer?
A: Amylase
B: Protease
C: Lipase
D: Catalase
Correct Answer: B
Explanation: Protease enzymes in meat tenderizer break down proteins into smaller molecules, making the meat easier to chew.

53: What process allows enzymes to temporarily react with the substrate to form a covalent intermediate?
A: Lock and key model
B: Transition state stabilization
C: Covalent catalysis
D: Induced fit model
Correct Answer: C
Explanation: Covalent catalysis involves enzymes temporarily reacting with the substrate, forming a covalent intermediate to provide a lower energy transition state.

54: What term refers to enzymes’ binding sites that cause a change in enzyme conformation and activity?
A: Allosteric sites
B: Catalytic sites
C: Active sites
D: Binding sites
Correct Answer: A
Explanation: Allosteric sites are pockets on the enzyme that bind molecules, causing a conformational change that affects enzyme activity.

55: Which enzyme has an average error rate of less than 1 error in 100 million reactions in high-fidelity mammalian polymerases?
A: Amylase
B: Catalase
C: DNA polymerase
D: Protease
Correct Answer: C
Explanation: DNA polymerase has proofreading mechanisms resulting in an average error rate of less than 1 in 100 million reactions.

56: Which enzyme system do viruses use for releasing from cells during infection?
A: HIV integrase
B: Neuraminidase
C: DNA polymerase
D: Reverse transcriptase
Correct Answer: B
Explanation: The influenza virus neuraminidase enzyme system is used by viruses for releasing from cells during infection.

57: How many amino acids typically compose enzyme catalytic sites?
A: 10-15
B: 5-7
C: 2-4
D: 8-12
Correct Answer: C
Explanation: Only a small portion of the enzyme structure (around 2-4 amino acids) is directly involved in catalysis at the catalytic site.

58: Through what metabolic pathway does NADPH regeneration occur in cells?
A: Glycolysis
B: Citric acid cycle
C: Pentose phosphate pathway
D: Electron transport chain
Correct Answer: C
Explanation: NADPH is regenerated through the pentose phosphate pathway in cells.

59: What is the term for enzymes in the liver involved in drug metabolism?
A: Transferases
B: Kinases
C: Cytochrome P450 oxidases
D: Orotidine decarboxylases
Correct Answer: C
Explanation: Cytochrome P450 oxidases are a group of enzymes in the liver involved in drug metabolism.

60: Which Nobel Prize was awarded to scientists for demonstrating that pure proteins can be enzymes?
A: Nobel Prize in Physics
B: Nobel Prize in Medicine
C: Nobel Prize in Chemistry
D: Nobel Prize in Literature
Correct Answer: C
Explanation: John Howard Northrop, Wendell Meredith Stanley, and James B. Sumner were awarded the Nobel Prize in Chemistry for demonstrating that pure proteins can be enzymes.

Disclaimer: Please consult a physician before making any changes to your health regimen.

What is the best quiz for you business?

Quizzes are super effective for lead generation and selling products. Find the best quiz for your business by answering a few questions.

Take the quiz